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bglP [2018-07-26 14:06:49]
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bglP [2018-07-26 14:06:49]

beta-glucoside permease of the phosphotransferase system, EIIBCA of the PTS, Trigger enzyme, control of LicT activity
Locus
BSU39270
Isoelectric point
6.81
Molecular weight
64.52 kDa
Protein length
609 aa Sequence Blast
Gene length
1827 bp Sequence Blast
Function
beta-glucoside uptake and phosphorylation, control of LicT activity
Product
beta-glucoside permease of the phosphotransferase system
Essential
no
E.C.
2.7.1.69
Synonyms
sytA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,033,778 → 4,035,607

The protein

Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family

  • PTS permease, sucrose family PubMed
  • Paralogous protein(s)

    Localization

  • cell membrane PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpA: repression, (CcpA binding site overlaps -35 region) PubMed, in CcpA regulon
  • LicT: anti-termination, via LicT-dependent RNA switch, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HprK, in LicT regulon
  • Regulation

  • induced by salicin (LicT) PubMed
  • Additional information

  • An [ncRNA|search|antisense RNA] is predicted for 'bglP' PubMed
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    Biological materials

    Mutant

  • GP475 (erm), available in the Stülke lab
  • BKE39270 (ΔbglP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
  • BKK39270 (ΔbglP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
  • Expression vector

  • pGP1290 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab,
  • pGP1300 (expression of bglP in B. subtilis, in pBQ200), available in Stülke lab
  • LacZ fusion

  • pGP600 (in pAC6), available in Stülke lab
  • References

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